The level of soluble _expression_ for each target was scored according to estimated protein yield.
Co-_expression_ of subunits within hosts such as Escherichia coli and insect cells has become more and more amenable, even at the level of high-throughput projects. Of these, yeast and baculovirus-infected insect cells currently represent the _expression_ systems of choice for structural biologists. Until further notice, the government has instituted policies to protect the. In particular, the SPINE standard meets the precision required for automatic sample exchange and includes a cap that is identified by a two-dimensional datamatrix code as well as an optional vial.
Although important biologically, for the structural biologist disordered regions of proteins can be disastrous even preventing successful structure determination.
Papers on the crystallization of biological molecules will be accepted providing that these focus on new methods or other features that are of general importance or applicability.
Development areas were broken down into workpackages and this article gives an overview of ongoing activity in the bioinformatics workpackage.
Obtaining well diffracting crystals is one of the major bottlenecks in the structure-determination pipeline.
Refinements of previously known structures may be published if sufficient new information is presented.
The system is suitable for use in conventional laboratories or can be implemented in a medium- or high-throughput pipeline.
Co-_expression_ of subunits within hosts such as Escherichia coli and insect cells has become more and more amenable, even at the level of high-throughput projects. Some individual projects and developments are discussed in detail, while those that are covered elsewhere in this issue are treated more briefly.
The inclusion of NMR spectroscopy in structural proteomics pipelines improves the success rate for protein structure determinations. SPINE was able to bring together developers of software with users from the partner laboratories. The technological developments and lessons learned from this project are discussed.
The city offers many temptations, but one of the toughest to avoid is peer pressure to drink.
Six human proteins were used to optimize the GRETA fermentation processes and to compare these processes with typical baffled-flask protocols used in structural genomics projects. Papers on the crystallization of biological molecules will be accepted providing that these focus on new methods or other features that are of general importance or applicability. Results are also reported from various case studies investigating different methods for performing co-_expression_ in E.