The level of soluble _expression_ for each target was scored according to estimated protein yield. The level of soluble _expression_ for each target was scored according to estimated protein yield.
Overall, HTP _expression_ screening in E.
Suitable vectors for HTP cloning are described and results from their use in _expression_ screening and protein-production pipelines are reported.
Of the eight soluble proteins, five were crystallized using robotic nanolitre-drop crystallization trials, which led to four solved structures.
The level of soluble _expression_ for each target was scored according to estimated protein yield.
Development areas were broken down into workpackages and this article gives an overview of ongoing activity in the bioinformatics workpackage. Refinements of previously known structures may be published if sufficient new information is presented. The level of soluble _expression_ for each target was scored according to estimated protein yield.
Six human proteins were used to optimize the GRETA fermentation processes and to compare these processes with typical baffled-flask protocols used in structural genomics projects. Worship ist Lobgesang und Stille Zeit zugleich - besinnlich und anregend. SPINE was able to bring together developers of software with users from the partner laboratories. Reports on new protein structures are particularly encouraged, as are papers on crystallographic binding studies, structural analysis of mutants and other structure-function studies.
Worship ist Lobgesang und Stille Zeit zugleich - besinnlich und anregend. coli and insect cells. Worship ist Lobgesang und Stille Zeit zugleich - besinnlich und anregend.
The accurate prediction of disorder is therefore important, not least for directing the design of _expression_ constructs so as to maximize the chances of successful structure determination.
Worship ist Lobgesang und Stille Zeit zugleich - besinnlich und anregend. This paper assesses the current state of the art in disorder prediction in terms of prediction reliability and considers how best to use these methods to guide construct design. Therefore, screening for soluble _expression_ on a small scale is an attractive way of identifying constructs that are likely to be amenable to structural analysis.
Often, this is caused by the poor quality of the protein preparation used for crystallization trials.
Some individual projects and developments are discussed in detail, while those that are covered elsewhere in this issue are treated more briefly.
This paper assesses the current state of the art in disorder prediction in terms of prediction reliability and considers how best to use these methods to guide construct design. The accurate prediction of disorder is therefore important, not least for directing the design of _expression_ constructs so as to maximize the chances of successful structure determination.