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Re: [PATCH] Add preseq.
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From: |
Ricardo Wurmus |
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Subject: |
Re: [PATCH] Add preseq. |
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Date: |
Thu, 25 Jun 2015 15:23:38 +0200 |
Attached is a new version that includes support for BAM files, but
without using the bundled version of samtools. Two patches were
necessary that I have submitted upstream.
I'm still testing this with a user, and I'm waiting for upstream to
comment on the patches, so I'm not in a hurry to push this.
~~ Ricardo
>From 3ffe6847848f6ba107e0757728f1c6a6e7807d56 Mon Sep 17 00:00:00 2001
From: Ricardo Wurmus <address@hidden>
Date: Tue, 23 Jun 2015 10:54:00 +0200
Subject: [PATCH] gnu: Add preseq.
* gnu/packages/bioinformatics.scm (preseq): New variable.
---
gnu/packages/bioinformatics.scm | 51 ++++++++++++++++++++++
.../patches/preseq-1.0.2-install-to-PREFIX.patch | 37 ++++++++++++++++
.../patches/preseq-1.0.2-link-with-libbam.patch | 33 ++++++++++++++
3 files changed, 121 insertions(+)
create mode 100644 gnu/packages/patches/preseq-1.0.2-install-to-PREFIX.patch
create mode 100644 gnu/packages/patches/preseq-1.0.2-link-with-libbam.patch
diff --git a/gnu/packages/bioinformatics.scm b/gnu/packages/bioinformatics.scm
index 4eb977b..d6fb32c 100644
--- a/gnu/packages/bioinformatics.scm
+++ b/gnu/packages/bioinformatics.scm
@@ -2033,6 +2033,57 @@ Databases are stored in a portable image within the file
system, and can be
accessed/downloaded on demand across HTTP.")
(license license:public-domain)))
+(define-public preseq
+ (package
+ (name "preseq")
+ (version "1.0.2")
+ (source (origin
+ (method url-fetch)
+ (uri
+ (string-append "http://smithlabresearch.org/downloads/preseq-";
+ version ".tar.bz2"))
+ (sha256
+ (base32 "0r7sw07p6nv8ygvc17gd78lisbw5336v3vhs86b5wv8mw3pwqksc"))
+ (patches (list (search-patch
"preseq-1.0.2-install-to-PREFIX.patch")
+ (search-patch
"preseq-1.0.2-link-with-libbam.patch")))
+ (modules '((guix build utils)))
+ (snippet
+ ;; Remove bundled samtools. Leave the headers.
+ '(for-each delete-file
+ (find-files "preseq-master/samtools"
+ "\\.c$")))))
+ (build-system gnu-build-system)
+ (arguments
+ `(#:tests? #f ;no "check" target
+ #:phases
+ (modify-phases %standard-phases
+ (add-after
+ 'unpack 'enter-dir
+ (lambda _
+ (chdir "preseq-master")
+ #t))
+ (delete 'configure))
+ #:make-flags (list (string-append "PREFIX="
+ (assoc-ref %outputs "out"))
+ (string-append "LIBBAM="
+ (assoc-ref %build-inputs "samtools")
+ "/lib/libbam.a"))))
+ (inputs
+ `(("gsl" ,gsl)
+ ("samtools" ,samtools-0.1)
+ ("zlib" ,zlib)))
+ (home-page "http://smithlabresearch.org/software/preseq/";)
+ (synopsis "Program for analyzing library complexity")
+ (description
+ "The preseq package is aimed at predicting and estimating the complexity
+of a genomic sequencing library, equivalent to predicting and estimating the
+number of redundant reads from a given sequencing depth and how many will be
+expected from additional sequencing using an initial sequencing experiment.
+The estimates can then be used to examine the utility of further sequencing,
+optimize the sequencing depth, or to screen multiple libraries to avoid low
+complexity samples.")
+ (license license:gpl3+)))
+
(define-public sra-tools
(package
(name "sra-tools")
diff --git a/gnu/packages/patches/preseq-1.0.2-install-to-PREFIX.patch
b/gnu/packages/patches/preseq-1.0.2-install-to-PREFIX.patch
new file mode 100644
index 0000000..f8318ae
--- /dev/null
+++ b/gnu/packages/patches/preseq-1.0.2-install-to-PREFIX.patch
@@ -0,0 +1,37 @@
+This patch has been submitted upstream:
https://github.com/smithlabcode/preseq/pull/15
+
+From 65387b0d766e6c06a15cf8d8f9183d9a4ba644bb Mon Sep 17 00:00:00 2001
+From: Ricardo Wurmus <address@hidden>
+Date: Thu, 25 Jun 2015 15:01:53 +0200
+Subject: [PATCH] Install to PREFIX
+
+---
+ Makefile | 8 ++++++--
+ 1 file changed, 6 insertions(+), 2 deletions(-)
+
+diff --git a/Makefile b/Makefile
+index 4d0367a..e334f2f 100644
+--- a/preseq-master/Makefile
++++ b/preseq-master/Makefile
+@@ -22,6 +22,10 @@ ifndef ROOT
+ ROOT = $(shell pwd)
+ endif
+
++ifndef PREFIX
++PREFIX = $(ROOT)
++endif
++
+ ifndef SMITHLAB_CPP
+ SMITHLAB_CPP=$(ROOT)/smithlab_cpp/
+ endif
+@@ -91,8 +95,8 @@ endif
+ $(CXX) $(CXXFLAGS) -o $@ $^ $(INCLUDEARGS) $(LIBS)
+
+ install: $(PROGS)
+- @mkdir -p $(ROOT)/bin
+- @install -m 755 $(PROGS) $(ROOT)/bin
++ @mkdir -p $(PREFIX)/bin
++ @install -m 755 $(PROGS) $(PREFIX)/bin
+
+ clean:
+ @-rm -f $(PROGS) *.o *~
diff --git a/gnu/packages/patches/preseq-1.0.2-link-with-libbam.patch
b/gnu/packages/patches/preseq-1.0.2-link-with-libbam.patch
new file mode 100644
index 0000000..035a3b1
--- /dev/null
+++ b/gnu/packages/patches/preseq-1.0.2-link-with-libbam.patch
@@ -0,0 +1,33 @@
+This patch has been submitted upstream:
https://github.com/smithlabcode/preseq/pull/15
+
+From a937a54d56e8a6ff06237471e568154d1fa5f267 Mon Sep 17 00:00:00 2001
+From: Ricardo Wurmus <address@hidden>
+Date: Thu, 25 Jun 2015 15:02:35 +0200
+Subject: [PATCH] Link with libbam.a if provided via LIBBAM
+
+---
+ Makefile | 7 +++++++
+ 1 file changed, 7 insertions(+)
+
+diff --git a/Makefile b/Makefile
+index e334f2f..de310e4 100644
+--- a/preseq-master/Makefile
++++ b/preseq-master/Makefile
+@@ -83,10 +83,17 @@ $(PROGS): $(addprefix $(SMITHLAB_CPP)/, \
+ preseq: continued_fraction.o load_data_for_complexity.o moment_sequence.o
+
+ ifdef SAMTOOLS_DIR
++ifdef LIBBAM
++LIBS += -pthread
++bam2mr preseq: $(addprefix $(SMITHLAB_CPP)/, SAM.o) \
++ $(LIBBAM)
++else
+ bam2mr preseq: $(addprefix $(SMITHLAB_CPP)/, SAM.o) \
+ $(addprefix $(SAMTOOLS_DIR)/, sam.o bam.o bam_import.o bam_pileup.o \
+ faidx.o bam_aux.o kstring.o knetfile.o sam_header.o razf.o bgzf.o)
+ endif
++endif # SAMTOOLS_DIR
++
+
+ %.o: %.cpp %.hpp
+ $(CXX) $(CXXFLAGS) -c -o $@ $< $(INCLUDEARGS)
--
2.1.0